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cells  (PromoCell)


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    PromoCell cells
    Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endothelial+cell+basal+medium+mv+2/Endothelial+Cell+Basal+Medium+MV+2%2C+phenol+red-free/pmc13135260-200-5-15
    Average 95 stars, based on 31 article reviews
    cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: ERG is a regulator of dynamic and reversible endothelial plasticity
    Article Snippet: yopreserved in BAMbanker freezing media (Fujifilm cellular dynamics CS-02–001) using a Mr. Frosty (Thermo Scientific™ N51000001). On the day of ATAC-seq, cells were washed with 1 mL of 37 °C ECGM-MV2 (PromoCell C-22221) and pelleted at 500 g. Free DNA from cells lysed during the cryopreservation process was removed through a 30 min incubation in ECGM-MV2 supplemented with 200 units of DNaseI (Therm

    Mouse Assay:

    Article Title: ERG is a regulator of dynamic and reversible endothelial plasticity
    Article Snippet: yopreserved in BAMbanker freezing media (Fujifilm cellular dynamics CS-02–001) using a Mr. Frosty (Thermo Scientific™ N51000001). On the day of ATAC-seq, cells were washed with 1 mL of 37 °C ECGM-MV2 (PromoCell C-22221) and pelleted at 500 g. Free DNA from cells lysed during the cryopreservation process was removed through a 30 min incubation in ECGM-MV2 supplemented with 200 units of DNaseI (Therm

    Modification:

    Article Title: ERG is a regulator of dynamic and reversible endothelial plasticity
    Article Snippet: yopreserved in BAMbanker freezing media (Fujifilm cellular dynamics CS-02–001) using a Mr. Frosty (Thermo Scientific™ N51000001). On the day of ATAC-seq, cells were washed with 1 mL of 37 °C ECGM-MV2 (PromoCell C-22221) and pelleted at 500 g. Free DNA from cells lysed during the cryopreservation process was removed through a 30 min incubation in ECGM-MV2 supplemented with 200 units of DNaseI (Therm



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    Image Search Results


    Cox4i2 is predominantly expressed in pericytes, smooth muscle cells and fibroblasts a) Schematic illustration of sample acquisition and data generation from CD45 + and CD45 - cells for single-cell RNAseq analyses. b) UMAP representation of CD45 - cells ( n =2–3 per group). c) UMAP highlighting Cox4i2 expressing cells in the pericyte/ smooth muscle cells (SMC) cluster of CD45 - cells. d) DotPlot visualization of marker genes for pericytes, SMCs and fibroblasts (FBs). e) Protein expression of COX4I2 in primary lung FBs, pulmonary artery SMCs (PASMCs), alveolar type II cells (ATIIs), endothelial cells (ECs), pericyte pellet, T cells, neutrophils and macrophages. PASMCs isolated from Cox4i2 −/− mice served as a negative control. f) Track plot of pericyte markers and fibroblast markers g) UMAP representation of the expression of Cox4i2 , Acta2 , or both in pericytes. Red: cells expressing Acta2 ; green: cells expressing Cox4i2 ; yellow: cells expressing both Acta2 and Cox4i2 . h) DotPlot visualization of marker genes expressed in pericytes. i) Labelling of Cox4i2 -expressing cells was performed using Cox4i2 -CreERT2-tdTomato mice. A Kozak-CreERT2-P2A cassette was inserted just before the Cox4i2 start codon, and these mice were crossed with tdTomato reporter mice containing a loxP-STOP-loxP cassette. After tamoxifen (i.p.; TAM) injection, Cre activation removed the stop cassette, enabling tdTomato expression driven by the Cox4i2 promoter. j, k) Representative images. j: tdTomato-positive cells in alveolar structures costained with CD31 as an endothelial marker and ACTA2 as an SMC marker. k: tdTomato-positive cells in pulmonary vessels. Colour code: Red – tdTomato, green – ACTA2, bright cyan – CD31, blue – Hoechst.

    Journal: bioRxiv

    Article Title: Mitochondrial COX4I2 drives pericyte-dependent inflammation and emphysema

    doi: 10.64898/2026.02.09.703513

    Figure Lengend Snippet: Cox4i2 is predominantly expressed in pericytes, smooth muscle cells and fibroblasts a) Schematic illustration of sample acquisition and data generation from CD45 + and CD45 - cells for single-cell RNAseq analyses. b) UMAP representation of CD45 - cells ( n =2–3 per group). c) UMAP highlighting Cox4i2 expressing cells in the pericyte/ smooth muscle cells (SMC) cluster of CD45 - cells. d) DotPlot visualization of marker genes for pericytes, SMCs and fibroblasts (FBs). e) Protein expression of COX4I2 in primary lung FBs, pulmonary artery SMCs (PASMCs), alveolar type II cells (ATIIs), endothelial cells (ECs), pericyte pellet, T cells, neutrophils and macrophages. PASMCs isolated from Cox4i2 −/− mice served as a negative control. f) Track plot of pericyte markers and fibroblast markers g) UMAP representation of the expression of Cox4i2 , Acta2 , or both in pericytes. Red: cells expressing Acta2 ; green: cells expressing Cox4i2 ; yellow: cells expressing both Acta2 and Cox4i2 . h) DotPlot visualization of marker genes expressed in pericytes. i) Labelling of Cox4i2 -expressing cells was performed using Cox4i2 -CreERT2-tdTomato mice. A Kozak-CreERT2-P2A cassette was inserted just before the Cox4i2 start codon, and these mice were crossed with tdTomato reporter mice containing a loxP-STOP-loxP cassette. After tamoxifen (i.p.; TAM) injection, Cre activation removed the stop cassette, enabling tdTomato expression driven by the Cox4i2 promoter. j, k) Representative images. j: tdTomato-positive cells in alveolar structures costained with CD31 as an endothelial marker and ACTA2 as an SMC marker. k: tdTomato-positive cells in pulmonary vessels. Colour code: Red – tdTomato, green – ACTA2, bright cyan – CD31, blue – Hoechst.

    Article Snippet: Primary endothelial cells (3□×□10□/mL) in MV-2 basal medium (Promocell) with 0.4% heparin, 0.1% ascorbic acid, and 0.1% hydrocortisone were seeded at 15,000 cells/well.

    Techniques: Single Cell, RNA sequencing, Expressing, Marker, Isolation, Negative Control, Injection, Activation Assay